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KMID : 0352519920290010219
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Korea Univercity Medical Journal
1992 Volume.29 No. 1 p.219 ~ p.232
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The Correlative Study on Expression of PCNA, EGFR, ER, C-myc Protein and Histopathologic Prognostic Factors in Uterine Cervical Carcinoma
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Abstract
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Among the various approaches for evaluating the proliferative activity. proliferating cell nuclear antigen (PCNA) has been recently introduced as an antigenic marker of cellular proliferation. Also, the level of the epidermal growth factor
receptor
(EGFR). estrogen receptor(ER) and c-myc protein has been known to correlate with the rates of cellular proliferation and DNA synthesis
In this study the expression of PCNA, EGFR, ER and c-myc protein was immunohistochemically evaluated and compared with the degree of malignancy, clinical stage and histologic subtypes using 44 cases of rountinely processed. formalin fixed
paraffin
sections of hysterectomized uterine cervical squamous cell carcinomas : 13 cases of in situ carcinomas 10 microinvasive carcinomas and 21 invasive carcinomas which were confined to the uterine cervical wall.
@ES The results were summerized as follows
@EN 1. The positivities of PCNA were 73.96±23.72%, 79.50±13.22%, and 71.90±22.61% in situ, microinvasive, and invasive squamous cell carcinoma. respectively. The difference was statistically insignificant.
2. There was no significant difference between the PCNA positivities of high grade malignacy group (72.50±19.73%).
3. Non-keratinizing type of carcinoma revealed significantly higher value of PCNA(84.44±8.46%) than keratinizing type (62.50±25.54%).
4. The staining indices of EGFR and c-myc protein in situ(0.92±0.57 and 0.76±0.62) and microinvasive(0.97±0.34 and 0.59±0.41) carcinomas were lower than in invasive carcinoma (1.23±0.75 and 1.80±0.80).
5. EGFR and c-myc protein in high grade malignancy group(1.24±0.71 and 1.06±0.78) showed significantly higher value of staining index than in low grade malignancy group(0.94±0.48 and 0.65±0.52).
6. In keratinizing type, the staining indices of EGFR and c-myc protein(1.61±0.67 and 1.33±0.94) were higher than in non-keratinizing type(0.83±0.62 and 0.75±0.45).
7. The staining index of ER was lower in invasive carcinoma(0.97±0.78) than in n situ and microinvasive carcinomas(1.42±0.78 and 1.59±0.75). And it was higher in low grade malignancy group(1.49±0.87) than in high grade malignancy
group(1.04±0.76).
8. The positivity of PCNA was not correlated with the staining indices of EGFR. ER and c-myc protein. However, there was positive correlation between the indices of EGFR and c-myc protein, which were negatively correlated with the index of ER.
From the above results, it is clear that the PCNA staining is useful as a proliferative marker which can be applied in routinely processed tissue sections and the expression of EGFR, ER and c-myc protein in squamous cell carcinoma can be used in
evaluation of grade.
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KEYWORD
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